Thrombopoiesis and thrombopoietin: the significance of "non-Tpo" cytokines.

Thrombopoiesis and Thrombopoietin: The Significance of ”non-Tpo“ Cytokines

Effect of thrombopoietin on the development of megakaryocytes and platelets: an ultrastructural analysis.

Megakaryocytopoiesis and platelet production can be assessed with reasonable accuracy by quantitative and functional analyses of circulating platelets. The evaluation of megakaryocytopoiesis in culture has remained unsatisfactory, particularly because platelet production is rarely observed. In mouse culture systems, megakaryocytes have been identified almost entirely by measurements of acetyl c...

Review article: pharmacological approaches for the treatment of thrombocytopenia in patients with chronic liver disease and hepatitis C infection.

BACKGROUND Patients with chronic liver disease and hepatitis C virus (HCV) frequently experience thrombocytopenia that complicates the management of their disease. Traditional therapy for thrombocytopenia consists of platelet transfusion, which can be associated with significant safety and economic issues. Consequently, efforts have been directed toward developing novel approaches for the treat...

The effects of thrombopoietin on megakaryocyte-cfc, megakaryocytes, and thrombopoiesis: with studies of ploidy and platelet size.

The effects of partially purified thrombopoietin (TPO). prepared from the plasma of thrombocytopenic rabbits, upon megakaryocyte colony-forming cells (Meg-CFC), megakaryocytes, and platelets were evaluated. The thrombopoiesis-stimulating activity of both types of preparations of TPO used was established by their ability to increase the levels of selenomethionine-75Se (75SeM) in the circulating ...

Thrombopoietin (c-mpl Ligand) Acts Synergistically With Erythropoietin, Stem Cell Factor, and Interleukin-l1 to Enhance Murine Megakaryocyte Colony Growth and Increases Megakaryocyte Ploidy In Vitro

Thrombopoietin (Tpo), the ligand for the ompl receptor, is a major regulator of platelet production in vivo. Treatment of mice with purified recombinant Tpo increases platelet count fourfold and expands colony-forming unit-megakaryocyte (CFU-Meg) numbers. Other cytokines including interleukin-3 (IL-3). IL-6, IL-11, erythropoietin (Epo), and stem cell factor (SCF) can stimulate megakaryopoiesis....